Fungal Image Gallery

The movie files provided on this page are in AVI format. For computers with slower network connections, the movies are also available in the compressed ZIP file format. None of the movies have sound.

Figure 1

Frame from Fig. 1 movie

• Download Fig. 1 (AVI, 51 MB)
• Download Fig. 1 (ZIP, 29.3 MB)

Figure 2

Frame from Fig. 2 movie

• Download Fig. 2 (AVI, 20 MB)
• Download Fig. 2 (ZIP, 13.3 MB)

Figure 3

Frame from Fig. 3 movie

• Download Fig. 3 (AVI, 20 MB)
• Download Fig. 3 (ZIP, 7.4 MB)

Figure 4

Frame from Fig. 4 movie

• Download Fig. 4 (AVI, 6.2 MB)
• Download Fig. 4 (ZIP, 2.1 MB)

Figure 5

Frame from Fig. 5 movie

• Download Fig. 5 (AVI, 6.2 MB)
• Download Fig. 5 (ZIP, 1.8 MB)

Figure 6

Frame from Fig. 6 movie

• Download Fig. 6 (AVI, 6.1 MB)
• Download Fig. 6 (ZIP, 4.2 MB)

Figure 7

Frame from Fig. 7 movie

• Download Fig. 7 (AVI, 17.1 MB)
• Download Fig. 7 (ZIP, 6.4 MB)

Figure 8

Frame from Fig. 8 movie

• Download Fig. 8 (AVI, 34.4 MB)
• Download Fig. 8 (ZIP, 14.2 MB)

Figure 9

Frame from Fig. 9 movie

Single optical sections from living hyphal tip cells of MG156 expressing BenR::EYFP. Apical region during interphase illustrated fluorescent-tagged microtubules and general cytoplasmic fluorescence. Time series of a nucleus during mitosis with a continual capture rate of 10 sec per frame. An increase in brightness occurred within the nucleus at the onset of mitosis preceding mitotic spindle initiation. The spindle was initially oriented perpendicular to the longitudinal axis of the hypha, but then rotated to become parallel during anaphase elongation. Astral and non-astral cytoplasmic microtubules are prominent throughout the time sequence with a reduction in the width of the central spindle as mitosis progressed.

Movie dimensions are 838x73, which may exceed the width of some monitors.

• Download Fig. 9 (AVI, 19.7 MB)
• Download Fig. 9 (ZIP, 5.4 MB)

Figure 10

Frame from Fig. 10 movie

Time series of single optical sections from a germ tube apex during treatment with 10 μg/ml griseofulvin with a continual capture rate of 12.5 sec per frame. Griseofulvin was applied immediately after the first frame was acquired. As microtubules depolymerized, aggregates of bright fluorescence developed typically with a larger aggregate forming near the apex. Apical aggregates exhibited peripheral fine filamentous elements that were oriented in a radial pattern. After 7.5 min of treatment, a prominent fluorescent aggregate at the apex was observed. Linear microtubule profiles were still visible after more than 20 min of treatment.

• Download Fig. 10 (AVI, 16.2 MB)
• Download Fig. 10 (ZIP, 3.1 MB)

Figure 11

Frame from Fig. 11 movie

Treatment of a mitotic nucleus with 20 μg/ml griseofulvin inhibited the formation of astral microtubule arrays, and caused the two half spindles to separate without pole-to-pole elongation. This cell was imaged with a continual capture rate of 3.2 sec per frame.

• Download Fig. 11 (AVI, 19.5 MB)
• Download Fig. 11 (ZIP, 2.7 MB)

Figure 12

Frame from Fig. 12 movie

A low magnification time-series of cytoplasmic ZsGreen expression in Fusarium. Vacuoles mitochondria and interphase nuclei exclude the fluorescent protein to varying degrees allowing details of their movement and dynamics to be revealed. This image was continually captured at a rate of 18 sec per frame.

Movie dimensions are 1827x487, which may exceed the width of some monitors.

• Download Fig. 12 (AVI, 61.9 MB)
• Download Fig. 12 (ZIP, 8.7 MB)

Figure 13

Frame from Fig. 13 movie

A high magnification time-series view of Fig. 4 documenting sporulation. Spherical interphase nuclei which partially exclude the fluorescent protein can be observed in conidiophores and the more mature binucleated spore. This image was continually captured at a rate of 18 sec per frame.

• Download Fig. 13 (AVI, 7.5 MB)
• Download Fig. 13 (ZIP, 1.8 MB)

Figure 14

Frame from Fig. 14 movie

A low magnification time-series of cytoplasmic ZsGreen expression in Magnaporthe griseae. This distil region of the tip cell documents the dynamic nature of numerous vacuoles. This image was continually captured at a rate of 12 sec per frame.

Movie dimensions are 2028x137, which may exceed the width of some monitors.

• Download Fig. 14 (AVI, 31.2 MB)
• Download Fig. 14 (ZIP, 13.4 MB)

Figure 15

Frame from Fig. 15 movie

A low magnification time-series of cytoplasmic ZsGreen expression in Magnaporthe griseae. The spitzenkoper of this tip cell and thread-like mitochondria are readily observed during hyphal elongation. This image was continually captured at a rate of 12 sec per frame.

• Download Fig. 15 (AVI, 11.8 MB)
• Download Fig. 15 (ZIP, 5.3 MB)

Figure 16

Frame from Fig. 16 movie

A high magnification time-series of cytoplasmic ZsGreen expression in Fusarium. Nuclear division can be readily followed as this multi-nucleate cell shows partial exclusion of ZsGreen during interphase and then rapid uptake of fluorescent protein at the onset of mitosis in a wave-like fashion. This image was continually captured at a rate of 8 sec per frame.

• Download Fig. 16 (AVI, 11.1 MB)
• Download Fig. 16 (ZIP, 5.8 MB)

Figure 17

Localization of gamma tubulin associated with the spindle pole body (arrows) of interphase nuclei (green) in the fungus Botrytis sp.

Figure 18

EYFP expressing fungus Magnaporthe griseae (green) penetrating a spongy mesophyl cell in Barley. The displacement of a chloroplast (red) occurred as a rigid penetration peg entered the host cell during a compatible interaction. Each frame in the movie represents a 6 minute 15 second interval.

• Download Fig. 18 (AVI, 744 KB)
• Download Fig. 18 (ZIP, 145 KB)

Figure 19

EYFP expressing fungus Magnaporthe griseae (green) penetrating a spongy mesophyl cell in Barley. Turgor pressure of the host cell was lost during fungal penetration in this compatible interaction. In addition, a rapidly moving intercellular hyphae grew past the infected cell. Each frame in the movie represents a 6 minute 15 second interval.

• Download Fig. 19 (AVI, 677 KB)
• Download Fig. 19 (ZIP, 101 KB)

Figure 20

Frame from Fig. 20 movie

A red-blue stereo anaglyph movie of cytoplasmic EYFP expression in Magnaporthe griseae demonstrating penetration and ramification within the epidermal cells of Barley. Each frame in the movie represented a one-hour interval starting 24 hours post inoculation.

• Download Fig. 20 (AVI, 13.5 MB)
• Download Fig. 20 (ZIP, 2.3 MB)