Confocal Microscope (Located in 304 Wolf Hall)

Confocal Microscope

• Zeiss LSM 510 VIS attached to an Axiovert 100M

Learn more about the basics of confocal microscopy

Specifications

Lasers

• ArKr laser (30 mW) with 488nm and 568nm excitation lines
• HeNe laser (5 mW) with 633nm excitation line
• Laser beam attenuation for all laser lines with a visible light AOTF

Detectors

• 3 PMT confocal reflection/fluorescence channels with a single independently variable confocal pinhole for each confocal channel
• 1 PMT transmitted light Channel

Objective Lenses

• 10x Plan Neofluar (NA 0.3)
• 20x Plan Neofluar (NA 0.5)
• 40x Plan Neofluar (NA 1.3) oil immersion
• 63x C-Apochromat (NA 1.2) water immersion
• 100x Plan-Apochromat (NA 1.4) oil immersion, DIC