Oristian, D.S., Sloofman, L.G., Zhou, Z., Wang, L., Farach-Carson, M.C. and Catherine B. Kirn-Safran. 2009. Ribosomal protein L29/HIP deficiency delays osteogenesis and increases fragiligy of adult bone in mice. J Orthopædic Res. 27(1): 28-35.

Cover Photo:Immunostaining for early and late markers of chondrocytedifferentiation on consecutive sections: type II (A, B) and type X collagen (C, D). Arrows (C, D) delineate the length of hypertrophic zones. Arrowheads in (A) indicate the periphery of developing bone.

Patrick Ollivier, Andrew Bahrou, Sarah Marcus, Talisha Cox, Thomas Church and Thomas Hanson. 2008. Volatilization and Precipitation of Tellurium by Aerobic, Tellurite Resistant Marine Microbes. Appl. Environ. Microbiol. 74(23):7163-7173.

Cover Photo: Thin-section transmission electron micrograph (TEM) of Bacillus sp. strain 6A with precipitated tellurium nanostructures highlighted in light blue. Cell constituents are colored deep blue and green on an orange field. The original TEM image was viewed using a Zeiss CEM 902 at 80 kV and captured with a Soft Imaging System Mega View II camera. The image was false colored using Adobe Photoshop CS3. Photo courtesy of Shannon Modla.

Thimmaraju Rudrappa, Kirk J. Czymmek, Paul W. Par and Harsh P. Bais. 2008. Root-Secreted Malic Acid Recruits Beneficial Soil Bacteria1[C][W][OA].  Plant Physiology. 148(3):1547–1556.

Cover Photo: A confocal three-dimensional maximum intensity projection of Bacillus subtilis biofilm formation (green) on living Arabidopsis thaliana Col-0 root stained with Calcoflour White (blue). Bacteria and plant nuclei (green) stained green with vital nucleic acid stain Syto 13.

Linda Sequeira, Cara Dubyk, Tracy Riesenberger, Carlton Cooper and Kenneth Van Golen. 2008. Rho GTPases in PC-3 prostate cancer cell morphology, invasion and tumor cell diapedesis. Clin Exp Metastasis (2008) 25:569–579.

Cover Photo: Effect of Rho and Rac GTPases on BMEC binding and diapedesis. 3-D composite images of diapedesis of PC-3 cells transfected with either shScr control or shRac.  The shScr images were taken 45 min and the shRac at 60 min after adding the tumor cells to the BMEC layer.

Xiaochun Zhang and, Andrew V. Teplyakov. 2008. Adsorption of C60Buckminster Fullerenes on an 11-Amino-1-undecene-Covered Si(111) Substrate. Langmuir. 24(3):810-820.

Cover Photo: An illustration collected and created by Xiaochun Zhang presents the atomic force microscopy images of fullerene buckyball C₆₀ molecules attached to the mixed 11-amino-1-undecence and decene self-assembled monolayers (SAMS) on Si(111)substrate.

Kirk Czymmek, Melissa Fogg, Deborah Powell, James Sweigard, Sook-Young Park, Seogchan Kang.  2007. In vivo time-lapse documentation using confocal and multi-photon microscopy reveals the mechanisms of invasion into the Arabidopsis root vascular system by Fusarium oxysporum.  Fungal Genetics & Biology. 44:1011-1023.

Cover Photo: Reef coral fluorescent protein ZsGreen labeled Fusarium oxysporum (green) infection of the lateral root (red) of Arabidopsis susceptible ecotype Cape Verdi Island (Cvi-0) taken 10 days post-inoculation.  This three-dimensional shadow projection was created from 37 optical slices acquired at a 1 micron z-interval.

Veeco. Sample courtesy of Liz Adams and Randall Duncan. Microscopy and Analysis, The Americas, March 2007, 83.

Cover Photo: A network of actin fibers that make up the cytoskeleton of live MC3T3F osteoblast cells which are mononucleated cells responsible for bone formation. Cells were imaged on a Veeco Bioscope II atomic force microscope (AFM). Scan size 100µm.

Manisha Malik and Robert A. Sikes. April 2006. Biotechniques. 40 (4).

Cover Photo: Human bone marrow endothelial-1 (HBME-1) cells. Mitochondria (red) were labeled in living cells with MitoTracker® Red; a biotinylated phage display peptide (L13) was localized with streptavidin-conjugated Alexa Fluor™ 488 (green); and nuclei (blue) were labeled with DRAQ5™. Cells were imaged using a Zeiss 510 NLO VIS confocal microscope.

R.C. Wagner, K. Czymmek and F.E. Hossler. 2006. Confocal Microscopy, Computer Modeling and Qauntification of Glomerular Vascular Corrosion Casts. Microscopy and Microanalysis.12:262-268.

Cover Photo: Confocal microscope image of a casted but uncorroded rabbit kidney renal corpuscle. Red regions highlight capillaries and arterioles, while green regions are stained to show various cell nuclei. Full width = 188um.

Ronald R. Gomes Jr., Mary C. Farach-Carson, and Daniel D. Carson. 2004. Perlecan Functions in Chondrogenesis: Insights from in vitro and in vivo Models. Cells Tissues Organs. 176:79-86.

Cover Photo: P/n domain I-stimulated mesenchymal cess condensation and differentiation.

Stephanie A. Miller, Anissa J. Brown, Mary C. Farach-Carson, and Catherine B. Kirn-Safran. 2003. HIP/RPL29 down-regulation accompanies terminal chondrocyte differentiation. Differentiation. 71:322-336.

Cover Photo: Confocal image of the developing murine cartilage growth plate stained for perlecan (green), F-actin (red) and nuclei (blue).

S.R. Dziennik, E.B. Belcher, G.A. Barker, M.J. DeBergalis, S.E. Fernandez, and A.M. Lenhoff. 2003. Nondiffusive mechanisms enhance protein uptake rates in ion exchange particles. PNAS. 100:420-425. Copyright 2003 National Academy of Sciences, U.S.A.

Cover Photo: Laser scanning confocal microscopic image of fluorescently labeled hen egg lysozyme in cation exchange particles. The bright red rings indicate areas of higher protein concentration and occur only after initial protein uptake. These findings demonstrate that mechanisms other than diffusion contribute to protein transport in oppositely charged porous material.

Timothy M. Bourett, James A. Sweigard, Kirk J. Czymmek, Anne Carroll and Richard J. Howard. 2002. Reef coral fluorescent proteins for visualizing fungal pathogens. Fungal Genetics & Biology. 37:211-220. Copyright 2002, with permission from Elsevier.

Cover Photo: A spectral fingerprint-based image of RCFP-expressing live cells. A Zeiss META system was employed to differentiate between germinating conidia of F. verticilloides expressing AmCyan (blue), ZsGreen (green), and ZsYellow (red).

Kirk J. Czymmek, Timothy M. Bourett, James A. Sweigard, Anne Carroll and Richard J. Howard. 2002. Utility of cytoplasmic fluorescent proteins for live-cell imaging of Magnaporthe grisea in planta. Mycologia. 94:280-289. Copyright 2002 by The Mycological Society of America.

Cover Photo: Cytoplasmic expression of green fluorescent protein in Magnaporthe grisea (green) infecting barley leaf (blue), imaged by Multiphoton microscopy. Appressoria (red) exhibited strong signal after pulsed, near infrared laser excitation false color, maximum intensity projection of thirty optical sections.

Michael C. Rice, Michael Bruner, Kirk Czymmek and Eric B. Kmiec. 2001. In vitro and in vivo nucleotide exchange directed by chimeric RNA/DNA oligonucleotides in Saccharomyces cerevisae. Molecular Microbiology. 40:857-868.

Cover Photo: Gene repair of a poit mutation located within a fusion reporter gene construct directed within a fusion reporter gene construct directed by synthetic oligonucleotides in Saccharomyces cerevisiae. Correction enables the normal protein to bind a ligand and emit green fluorescence.