Mass Spectrometry Facility
Contact: John Dykins
302 831 3578
dykins@udel.edu
The mass Spec Core Facility is located in the Department of Chemistry & Biochemistry, Lammot duPont Lab at the University of Delaware in Newark DE.
The core facility is grouped into three areas; the first laboratory houses the high performance instruments for more detailed and precise analyses, a second lab houses three open-access user-friendly instruments that are available for researchers to use 24/7, and finally, a high-performance MALDI instrument is located in Dr. Murray V. Johnston’s Lab.
High Performance Instrumentation Lab
Room 122 Lammot duPont Lab
Protein sequencing
Micromass model Ultima Q Tof
(Quadrupole-Time of Flight tandem mass spectrometer).
This is an LC-MS-MS instrument primarily used to identify proteins by sequencing the products of a digested protein.
Product molecular ions and subsequent collisional activation to generate fragments are automatic. Software generated or de novo peptide sequencing can be compared to protein databases for identification.
Protein Digester
Bruker Daltonics model Proteineer DP
This automated digest and prep station performs protein digests.
Digest products can be automatically spotted to a MALDI sample plate or captured in MTP format for LC-MSMS analyses.
Open Access Instrumentation lab
Room 108 Lammot duPont Lab
GC-MS (Gas Chromatography-Mass Spectrometry)
Agilent models 6850 GC and 5973 MS with autosampler.
Used primarily for separation of small organic mixtures, incorporates a NIST library to aid identification.
LC-MS (Liquid Chromatography-Mass Spectrometry)
Thermo-Finnigan model LCQ using Electrospray Ionisation (ESI) with an integrated autosampler.
Used for biological samples and polar organics. The instrument can be switched between column mode for mixture separations and loop injections for quick analyses of purer samples.
MALDI (Matrix Assisted Laser Desorption Ionisation)
Bruker Daltonics model Omniflex.
Used primarily for biological samples to determine molecular weight and purity.
Dr. Murray V. Johnston Lab
Room 125 Lammot duPont Lab
MALDI
Bruker Daltonics model Biflex MALDI mass spectrometer.
Used for high resolution and accurate mass measurements; automated protein identification via peptide mass maps.
