Protein Production Facility
DBI Rooms 136, 147 and 239

Contact: Dr. Yu-Sung Wu
(302) 831-4687
wu@dbi.udel.edu

Scheduling

The Protein Production Core Facility at DBI provides fermentation and purification capabilities for large-scale protein expression and purification. Two New Brunswick bioreactors, BioFlo 4500 for bacterial and yeast cells, and Celligen Plus for insect and mammalian cells, can deliver up to 22 L volumes for cell growth. The facility also includes equipment for cell rupture and for protein purification and characterization. The installation was established and is operated with the support of the National Center for Research Resources of the National Institutes of Health as part of the COBRE in Structural and Functional Genomics at the University of Delaware.

• New Brunswick BioFlo 4500 fermentor (30 L capacity for bacterial and yeast cell growth)
• New Brunswick Celligen Plus Bioreactor (7.5 L capacity for insect and mammalian cell growth)
• Avestin EmulsiFlex C5 high pressure homogenizer
• Amersham Pharmacia Akta Explorer 100 high performance liquid chromatography system
• Beckman Coulter JLA 8.1000 centrifuge rotor
• Amicon stirred ultrafiltration cell
• Invitrogen Xcell protein gel electrophoresis apparatu